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Updated: May 15, 2026

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
Published on: August 9, 2022
Determining the yield of RNA synthesized in vitro
Abstract:
A simple way to determine the amount of RNA made by in vitro transcription is to include a trace amount of radioactive nucleotide and calculate the amount of nucleotides incorporated into the RNA, as described here. This protocol can also be used to quantitate unlabeled transcripts, assuming that the unlabeled RNA is equivalent to an aliquot of labeled RNA synthesized in parallel.
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