Related Experiment Video
Updated: May 15, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: March 8, 2020
Enantioseparations by high-performance liquid chromatography using polysaccharide-based chiral stationary phases: an
1Institute of Physical and Analytical Chemistry, Tbilisi State University, Tbilisi, Georgia. bezhan_chankvetadze@yahoo.com
Abstract:
This chapter summarizes the application of polysaccharide-based chiral stationary phases (CSPs) for separation of enantiomers in high-performance liquid chromatography (HPLC). Since this book contains dedicated chapters on enantioseparations using supercritical fluid chromatography (SFC), capillary electrochromatography (CEC), and simulated moving bed (SMB) chromatography, the application of polysaccharide-based materials in these modes of liquid phase separation techniques are touched just superficially. More emphasis is directed toward a discussion of the optimization of polysaccharide-based chiral selectors, their attachment onto the carrier, and the optimization of the support. The optimization of the separation of enantiomers based on various parameters such as mobile phase composition and temperature is also discussed.
More Related Videos
09:57Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
Published on: June 14, 2020
07:25Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: January 5, 2021
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Ion-Exchange Chromatography
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
High-Performance Liquid Chromatography: Elution Process
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...