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Updated: Jan 26, 2026

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: January 5, 2021
Enantioselective high-performance liquid chromatography-tandem mass spectrometry method for bioanalysis and chemical
Saba Jorbenadze1, Tamar Khatiashvili1, Giorgia Sprega2
1Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, I. Chavchavadze Ave 1, Tbilisi 0179, Georgia.
Abstract:
The first high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method was developed for enantioselective analysis of psychoactive compound N-ethylpentedrone. The method also enabled a detection of phase-1 metabolites of N-ethylpentedrone. Enantiomers some of the phase-1 metabolites were also separated. Application of this method to urine and oral fluid samples from the controlled naturalistic clinical study indicated to no enantioselective metabolism and elimination of N-ethylpentedrone. With the developed method a certain chemical and stereochemical instability of N-ethylpentedrone was also shown. Therefore, the observation about non-enantioselective pharmacokinetics of N-ethylpentedrone can be apparent and caused by post-collection racemization of this compound in the studied biological matrices.
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