Related Experiment Videos
Effect of glucose upon alkaline picrate: a Jaffé interference
1Department of Chemistry, University of Regina, Saskatchewan, Canada.
Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|February 1, 1990
Summary
The reaction between glucose and alkaline picrate produces picramic acid, identified by spectrophotometry and polarography. This study details the chemical transformation and product identification.
Area of Science:
- Analytical Chemistry
- Spectroscopy
- Electrochemistry
Background:
- Alkaline picrate is a reagent used in various chemical analyses.
- Understanding the reactivity of carbohydrates with oxidizing agents is crucial in biochemistry and analytical chemistry.
Purpose of the Study:
- To investigate the reactivity of glucose in an alkaline picrate solution.
- To identify and characterize the reaction products using spectrophotometry, polarography, thin-layer chromatography, and infrared spectroscopy.
Main Methods:
- Spectrophotometry and polarography were employed to study the reaction kinetics.
- Thin-layer chromatography (TLC) and infrared (IR) spectroscopy were used for product identification.
- Reactions were conducted in aqueous sodium hydroxide at 25°C with varying glucose:picrate molar ratios.
Main Results:
- Picramic acid was conclusively identified as the major product in glucose:picrate solutions (5:1 and 10:1 molar ratios).
- Polarography showed decreased diffusion currents for reduction waves 1-3 upon glucose addition, indicating reaction.
- Spectra shifts and the formation of a fluorescent orange minor product were observed, alongside the red major product, picramic acid.
Conclusions:
- Glucose reacts with alkaline picrate to form picramic acid, with formation observed within 10 minutes.
- The reaction involves a color change from yellow to orange and then red.
- The study provides detailed analytical data on the glucose-picrate reaction products.