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Updated: May 15, 2026

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry
Published on: April 21, 2017
In-solution digestion of glycoproteins for glycopeptide-based mass analysis
Eden P Go1, Kathryn R Rebecchi, Heather Desaire
1Department of Chemistry, University of Kansas, Lawrence, KS, USA.
Abstract:
Glycopeptides are generated from the enzymatic digestion of glycoproteins with a specific or nonspecific protease. Whether this enzymatic conversion of glycoproteins into glycopeptides and peptides is done in-solution or in-gel, an efficient digestion protocol is one of the key components of a successful outcome in a mass spectrometry-based experimental workflow. This chapter outlines an optimized in-solution digestion protocol to prepare samples for glycopeptide-based mass analysis.

