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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

RAGE splicing variants in mammals.

Katharina Anna Sterenczak1, Ingo Nolte, Hugo Murua Escobar

  • 1Small Animal Clinic, University for Veterinary Medicine, Hannover, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|January 9, 2013
PubMed
Summary
This summary is machine-generated.

The receptor for advanced glycation end products (RAGE) and its variants are crucial in diseases. Canine RAGE shares similarities with human RAGE, offering potential for comparative therapeutic development.

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

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Last Updated: May 15, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Area of Science:

  • Molecular biology
  • Immunology
  • Genetics

Background:

  • The receptor for advanced glycation end products (RAGE) is a key mediator in various pathophysiological processes.
  • RAGE exhibits extensive splicing, producing numerous isoforms that can regulate full-length RAGE function.
  • Soluble RAGE isoforms are implicated in RAGE-associated disorders and are frequently measured in clinical settings.

Purpose of the Study:

  • To investigate the structural and functional similarities of canine RAGE to human RAGE.
  • To explore the potential of canine RAGE as a model for understanding RAGE-mediated diseases.
  • To lay the groundwork for developing RAGE-specific therapies applicable to both human and veterinary medicine.

Main Methods:

  • Comparative gene structure analysis between human and canine RAGE.
  • Identification and characterization of canine RAGE splicing variants.
  • Review of existing literature on RAGE and its isoforms in human and canine diseases.

Main Results:

  • Canine RAGE gene exhibits high structural similarity to human RAGE, indicating evolutionary conservation.
  • Canine RAGE demonstrates complex splicing activity, generating diverse isoforms similar to humans.
  • Similarities in RAGE-associated diseases between dogs and humans were noted.

Conclusions:

  • Canine RAGE serves as a valuable model for studying RAGE biology and associated pathologies.
  • Comparative analysis of RAGE in dogs and humans can inform the development of novel therapeutic strategies.
  • Targeting RAGE and its ligands holds promise for both human and veterinary medical applications.