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Updated: May 15, 2026
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Quantifying Yeast Chronological Life Span by Outgrowth of Aged Cells
Published on: May 6, 2009
Assessing chronological aging in Saccharomyces cerevisiae.
Jia Hu1, Min Wei, Mario G Mirisola
1Ethel Percy Andrus Gerontology Center, Davis School of Gerontology, University of Southern California, Los Angeles, CA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|January 9, 2013
Summary
This study explores aging in Saccharomyces cerevisiae using chronological lifespan (CLS) assays. It highlights methods to study yeast aging and macromolecular damage, focusing on genomic instability.
Area of Science:
- Gerontology and molecular biology
- Yeast as a model organism for aging research
Background:
- Saccharomyces cerevisiae is a key model for studying aging mechanisms.
- Chronological lifespan (CLS) assay is a primary method for yeast aging studies.
- Multiple assay methods are crucial to validate findings and minimize artifacts.
Purpose of the Study:
- To describe various methods for determining CLS in yeast.
- To detail assays for studying macromolecular damage during yeast aging.
- To focus on genomic instability as a key aging factor.
Main Methods:
- Detailed description of chronological lifespan (CLS) assays.
- Assays for assessing macromolecular damage in aging yeast.
- Methods specifically targeting genomic instability.
Main Results:
- Comprehensive overview of established and novel CLS assay techniques.
- Identification of key assays for evaluating aging-related damage.
- Emphasis on the role of genomic instability in yeast aging.
Conclusions:
- Multiple CLS assay methods enhance the reliability of aging research in yeast.
- Understanding yeast aging provides insights into aging in higher eukaryotes.
- Genomic instability is a critical factor in yeast aging processes.
