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Quantitative and multiplex detection of pathogenic fungi using padlock probes, generic qPCR, and suspension array

Magnus Jobs1, Ronnie Eriksson, Jonas Blomberg

  • 1School of Health and Social Studies, Dalarna University, Falun, Sweden. magnus.jobs@medsci.uu.se

Methods in Molecular Biology (Clifton, N.J.)
|January 9, 2013
PubMed

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Real Time RT-PCR02:57

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...