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Related Concept Videos

B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...

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Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
08:20

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

Engineering B cells with mRNA.

Jaewoo Lee1, David Boczkowski, Smita Nair

  • 1Department of Surgery, Duke University Medical Center, Durham, NC, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 9, 2013
PubMed
Summary
This summary is machine-generated.

Ex vivo activated B cells can be enhanced to act as potent antigen-presenting cells (APCs) for immunotherapy. Modifying B cells with specific mRNAs boosts their immune stimulation capabilities, offering an alternative to dendritic cells (DCs).

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Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Ex vivo activated B cells are explored as antigen-presenting cells (APCs).
  • Concerns exist regarding their potency compared to dendritic cells (DCs).

Purpose of the Study:

  • To enhance the antigen presentation and immune stimulation functions of activated B cells.
  • To develop activated B cells as a viable alternative to DCs in immunotherapy.

Main Methods:

  • Co-transfection of activated B cells with multiple mRNAs.
  • Encoding for costimulatory molecules (OX40L, 4-1BBL, CD80).
  • Encoding for cytokines (IL-12p35, IL-12p40) and antigen.

Main Results:

  • Modified B cells demonstrated enhanced antigen presentation.
  • Improved immune stimulation functions were observed.
  • These modified B cells show potential as APCs.

Conclusions:

  • A strategy was developed to modulate B cell APC function.
  • Modified B cells represent a potent alternative to DCs for immunotherapy.
  • This approach enhances B cell utility in therapeutic applications.