Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multistage nanomedicine engineering to overcome sequential barriers to glioblastoma treatment: a review.

Journal of nanobiotechnology·2026

Same author

Respiratory function profiling using non-invasive methods in children aged 7-18 years: a hospital based study from North India.

BMC pediatrics·2026

Same author

Unveiling a Hidden Conversion Pathway in CoSe<sub>2</sub> Anodes via Rationally Designed CNT-Interwoven Hollow Carbon Microclusters for High-Performance Potassium-Ion Batteries.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Negative cooperativity drives activity of capsid-directed antivirals against hepatitis B virus.

Science advances·2026

Same author

Incidence of Bacille Calmette-Guérin associated lymphadenitis in healthy children: A systematic review and meta-analysis.

Human vaccines & immunotherapeutics·2026

Same author

Stimuli-Responsive Zirconium-Based Metal-Organic Frameworks for Targeted Cancer Drug Delivery.

ACS applied materials & interfaces·2026

Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 15, 2026

Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation

Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation

Published on: July 8, 2011

Programming human dendritic cells with mRNA.

Jaewoo Lee¹, David Boczkowski, Smita Nair

¹Department of Surgery, Duke University Medical Center, Durham, NC, USA.

Methods in Molecular Biology (Clifton, N.J.)

|January 9, 2013

Summary

This summary is machine-generated.

Messenger RNA (mRNA) transfection effectively expresses proteins in primary cells. Electroporation is an efficient method for delivering mRNA into human dendritic cells (DCs) for cancer immunotherapy vaccines.

More Related Videos

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood

Published on: December 24, 2016

Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells In Vitro and In Vivo

Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells In Vitro and In Vivo

Published on: February 5, 2021

Related Experiment Videos

Last Updated: May 15, 2026

Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation

Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation

Published on: July 8, 2011

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood

Published on: December 24, 2016

Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells In Vitro and In Vivo

Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells In Vitro and In Vivo

Published on: February 5, 2021

Area of Science:

Immunology
Molecular Biology
Cellular Biology

Background:

Messenger RNA (mRNA) transfection is a versatile tool for protein expression in primary cells.
mRNA can encode antigens for antigen-presenting cell (APC) generation or immune-modulating molecules to program cell function.
Current methods focus on efficient mRNA delivery into APCs for therapeutic applications.

Purpose of the Study:

To describe an efficient method for delivering mRNA into human dendritic cells (DCs).
To highlight the utility of mRNA transfection in cellular immunotherapy and vaccine development.

Main Methods:

In vitro transcription of protein-encoding mRNA.
Electroporation as the delivery method for mRNA into human dendritic cells (DCs).
Utilizing antigen-encoding or immune-modulating molecule-encoding mRNA.

Main Results:

High levels of desired RNA-encoded proteins are expressed in primary cells following mRNA transfection.
mRNA-transfected DCs can be processed to generate antigen-presenting cells.
Electroporation provides an efficient route for mRNA delivery into human DCs.

Conclusions:

mRNA transfection is a powerful technique for protein expression and cellular programming.
Electroporation is the preferred method for delivering mRNA into APCs for cancer immunotherapy.
This approach holds significant promise for developing novel vaccines and immunotherapies.