Related Experiment Video
Updated: May 15, 2026
10:10
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
Published on: March 31, 2019
Chtop is a component of the dynamic TREX mRNA export complex
Chung-Te Chang1, Guillaume M Hautbergue, Matthew J Walsh
1Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK.
The EMBO Journal
|January 10, 2013
Summary
Researchers discovered Chtop as a new TREX complex component. Chtop and Alyref activate Uap56, crucial for mRNA export, revealing dynamic remodeling of the TREX-Nxf1 complex.
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- The TREX complex is essential for coupling mRNA processing with export.
- Key components include Uap56, Alyref, Cip29, and the THO complex.
Purpose of the Study:
- To identify novel components of the TREX complex.
- To elucidate the role of Chtop in mRNA export and its interaction with other factors.
Main Methods:
- Protein interaction studies (co-immunoprecipitation).
- Biochemical assays for ATPase and RNA helicase activity.
- RNA interference (RNAi) for gene knockdown.
- In vivo complex analysis.
Main Results:
- Chtop was identified as a novel TREX component.
- Chtop and Alyref activate Uap56's ATPase and RNA helicase activities.
- Chtop interacts with Nxf1, requiring arginine methylation.
- Co-knockdown of Alyref and Chtop caused a significant mRNA export block.
- TREX and Nxf1 components undergo dynamic remodeling.
Conclusions:
- Chtop is a functional component of the TREX complex, essential for mRNA export.
- The TREX-Nxf1 interaction is dynamic, regulated by Uap56 activity and Chtop modification.
More Related Videos
Related Concept Videos
01:24Protein Transport to the Stroma
Chloroplasts are triple membrane structures with an outer membrane, an inner membrane, and a thylakoid membrane, each containing distinct metabolite transporters, membrane translocons, and enzymes. Appropriate sorting and translocating these proteins to their correct membrane systems is essential for chloroplast function.
Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
02:41Protein Complex Assembly
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
02:41Protein Complex Assembly
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
02:35Transcription Elongation Factors
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
02:35Transcription Elongation Factors
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
01:30General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...