Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Differential Staining Technique01:26

Differential Staining Technique

Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Green Valorization of Two-Phase Olive Pomace via Pressurized Liquid Extraction: Process Optimization, Comprehensive Metabolite Analysis and Functional Applications.

Molecules (Basel, Switzerland)·2026
Same author

Membrane structural properties in Staphylococcus aureus are tuned by the carotenoid 4,4'-diaponeurosporenoic acid.

Biochimica et biophysica acta. Biomembranes·2026
Same author

Generation of human primordial germ cell-like cells from cumulus and blood cell-derived isogenic hiPSCs†.

Biology of reproduction·2026
Same author

Goals in Nutrition Science 2025-2030.

Frontiers in nutrition·2026
Same author

Monoterpenoid-Based Deep Eutectic Solvents/Natural Deep Eutectic Solvents: A Review on Its Characteristics and Applications.

ACS omega·2026
Same author

<i>Bombyx mori</i> Pupae as a Novel Ingredient from an Underutilized Sericulture Product: A Dual Approach Based on Sustainable Extractions and Sample Pretreatment Strategies.

ACS sustainable chemistry & engineering·2026

Related Experiment Video

Updated: May 15, 2026

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
11:25

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

Comparison of three differential mouse blastocyst staining methods.

Anna Mallol1, Josep Santaló, Elena Ibáñez

  • 1Departament de Biologia Cel·lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.

Systems Biology in Reproductive Medicine
|January 15, 2013
PubMed
Summary

Comparing differential staining methods for mouse blastocysts, researchers found that permeabilization and immunodetection techniques are superior for accurately counting trophectoderm (TE) and inner cell mass (ICM) cells.

More Related Videos

Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos
10:17

Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos

Published on: January 31, 2016

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology
13:05

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Published on: March 17, 2011

Related Experiment Videos

Last Updated: May 15, 2026

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
11:25

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos
10:17

Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos

Published on: January 31, 2016

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology
13:05

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Published on: March 17, 2011

Area of Science:

  • Embryology
  • Developmental Biology
  • Reproductive Science

Background:

  • Blastocyst quality assessment is crucial in reproductive technologies.
  • Differential staining is a common method for evaluating blastocyst cell lineages: trophectoderm (TE) and inner cell mass (ICM).

Purpose of the Study:

  • To compare the efficiency of three differential staining methods for mouse blastocysts.
  • To evaluate cell counting accuracy for TE, ICM, and total cells using different techniques.

Main Methods:

  • Differential staining of mouse blastocysts using three distinct methods.
  • Method 1: TE selective labeling with a lectin.
  • Method 2: TE permeabilization using a detergent.
  • Method 3: Immunodetection of TE and ICM specific markers.

Main Results:

  • TE permeabilization and immunodetection methods demonstrated superior efficiency.
  • Both superior methods provided equivalent counts for TE, ICM, and total cells.
  • Lectin-based TE selective labeling was less effective in comparison.

Conclusions:

  • TE permeabilization and immunodetection are reliable and accurate methods for blastocyst cell counting.
  • These methods enhance the evaluation of blastocyst quality in research settings.
  • Accurate cell counting is vital for understanding early embryonic development and improving assisted reproductive technologies.