A single mesoporous ZnO/Chitosan hybrid nanostructure for a novel free nanoprobe type biosensor

A novel method of fabricating a free probe type nanoscale biosensor is first demonstrated. A free probe type biosensor based on a single one-dimensional (1D) mesoporous ZnO/Chitosan inorganic-organic hybrid nanostructure is constructed. The as-prepared biosensor exhibited excellent biosensing performance and was stable in solution due to its inorganic-organic hybrid structure. The special mesoporous nanostructure with protuberances favors enzymes loading and enhances electrical communication efficiency. The feasibility of employing a single 1D nanostructure as a separate free nanoprobe for biosensing is demonstrated. This kind of free probe type biosensor will not only provides a new tool for micro-targets detection in microcell and enzymatic studies, but also has the potential to be inserted into single cell or other microorganism for biological studies.