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Optical Trapping of Nanoparticles
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Optical Trapping of Nanoparticles

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A trigger channel threshold artifact in nanoparticle analysis.

John P Nolan1, Samuel A Stoner

  • 1La Jolla Bioengineering Institute, San Diego, California 92037, USA. jnolan@ljbi.org

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|January 22, 2013
PubMed
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Analyzing dim nanoparticles with flow cytometry requires careful threshold settings. Incorrect trigger channel thresholds can lead to artifactual data, underestimating particle counts and brightness. Using a fluorescence channel for triggering is recommended for accurate dim nanoparticle analysis.

Area of Science:

  • Nanoparticle analysis
  • Flow cytometry
  • Analytical chemistry

Background:

  • Flow cytometry is used for individual nanoparticle analysis, often involving dim signals near instrument detection limits.
  • Distinguishing true nanoparticle signals from background noise and artifacts is a significant challenge.
  • Careful experimental design, control experiments, and data scrutiny are essential for reliable measurements.

Purpose of the Study:

  • To identify and explain an artifact arising from inappropriate trigger channel threshold selection in flow cytometry for dim nanoparticle analysis.
  • To demonstrate how incorrect threshold settings lead to erroneous measurements of particle number and brightness.
  • To propose an improved triggering strategy for accurate dim nanoparticle detection.

Main Methods:

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Last Updated: May 15, 2026

Optical Trapping of Nanoparticles
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Published on: January 15, 2013

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09:13

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment

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  • Analysis of flow cytometry data, focusing on the impact of trigger channel threshold settings on dim signal detection.
  • Comparison of data acquired using different triggering strategies (e.g., trigger channel vs. fluorescence channel).
  • Evaluation of artifactual events resulting from low signal detection near background levels.

Main Results:

  • An artifact caused by inappropriate trigger channel threshold adjustment was identified.
  • When particle signals fall below the trigger threshold, only coincident events are detected, leading to inaccurate quantification.
  • Erroneous measurements of both particle concentration and signal intensity (brightness) were observed under specific thresholding conditions.

Conclusions:

  • Inappropriate trigger channel threshold selection can produce significant artifacts in dim nanoparticle flow cytometry.
  • The common practice of minimizing 'false triggers' by raising the threshold can exclude true dim particles.
  • Utilizing a fluorescence channel for triggering is suggested as a more robust method for accurate dim nanoparticle analysis.