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Updated: May 14, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Uptake and processing of semiconductor quantum dots in living cells studied by fluorescence lifetime imaging

Lina Carlini1, Jay L Nadeau

  • 1Department of Biomedical Engineering, McGill University, 3775 University Street, Montreal, QC H3A 2B4, Canada.

Chemical Communications (Cambridge, England)
|January 25, 2013
PubMed

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...