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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

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Related Experiment Video

Updated: May 14, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
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Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

DMD-based LED-illumination super-resolution and optical sectioning microscopy.

Dan Dan1, Ming Lei, Baoli Yao

  • 1State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.

Scientific Reports
|January 25, 2013
PubMed
Summary
This summary is machine-generated.

This study introduces a cost-effective structured illumination microscopy (SIM) technique using a digital micromirror device (DMD) and LED illumination. It achieves 90 nm lateral resolution and 120 μm optical sectioning depth for live cell imaging.

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Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

Area of Science:

  • Biophysics
  • Optical Microscopy
  • Cell Biology

Background:

  • Super-resolution microscopy is crucial for studying live biological systems.
  • Existing high-resolution techniques like electron microscopy have limitations for live samples.
  • Structured Illumination Microscopy (SIM) offers a balance of resolution and live-cell compatibility.

Purpose of the Study:

  • To develop a novel, cost-effective, and versatile super-resolution 3D optical microscopy technique.
  • To enhance imaging speed and optical sectioning capabilities for live cell and tissue studies.
  • To overcome limitations of conventional SIM, such as cost and complexity.

Main Methods:

  • Implemented a digital micromirror device (DMD) for structured fringe projection.
  • Utilized low-coherence LED light for illumination.
  • Developed a system capable of switching between 2D super-resolution and 3D optical sectioning modalities.

Main Results:

  • Achieved a lateral resolution of 90 nm.
  • Reached an optical sectioning depth of 120 μm.
  • Demonstrated a maximum acquisition speed of 1.6×10(7) pixels/second in 3D mode.
  • The system is cost-effective, easily switchable for multi-wavelengths, and free from speckle noise.

Conclusions:

  • The DMD-based LED-illumination SIM is a powerful and accessible tool for high-resolution live imaging.
  • This technique offers significant advantages in cost, versatility, and noise reduction compared to other SIM methods.
  • It is applicable to both fluorescent and non-fluorescent specimens, broadening its utility in biological research.