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Related Experiment Video

Updated: May 14, 2026

FIM Imaging and FIMtrack: Two New Tools Allowing High-throughput and Cost Effective Locomotion Analysis
10:02

FIM Imaging and FIMtrack: Two New Tools Allowing High-throughput and Cost Effective Locomotion Analysis

Published on: December 24, 2014

FIM, a novel FTIR-based imaging method for high throughput locomotion analysis.

Benjamin Risse1, Silke Thomas, Nils Otto

  • 1Institute for Neurobiology, University of Münster, Münster, Germany.

Plos One
|January 26, 2013
PubMed
Summary

We developed a novel imaging technique (FTIR-based Imaging Method) for high-resolution biological movies. This method enhances high-throughput screening of complex behaviors in organisms like Drosophila larvae.

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Area of Science:

  • Biophysics
  • Neurobiology
  • Developmental Biology

Background:

  • High-resolution imaging is crucial for analyzing complex biological processes.
  • Current methods may lack the contrast or throughput for detailed behavioral analysis.
  • Understanding gene function in behavior requires sensitive screening tools.

Purpose of the Study:

  • To introduce a novel imaging technique for high-resolution, high-contrast movie acquisition.
  • To develop a robust tracking software for analyzing locomotion in crowded biological samples.
  • To demonstrate the utility of the method for high-throughput genetic screens of behavior.

Main Methods:

  • Frustrated Total Internal Reflection (FTIR)-based Imaging Method (FIM) for in vivo imaging across all wavelengths.

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Last Updated: May 14, 2026

FIM Imaging and FIMtrack: Two New Tools Allowing High-throughput and Cost Effective Locomotion Analysis
10:02

FIM Imaging and FIMtrack: Two New Tools Allowing High-throughput and Cost Effective Locomotion Analysis

Published on: December 24, 2014

Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury
07:28

Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury

Published on: March 24, 2023

  • Development of FIMTrack software with a module to maintain larval identity during collisions.
  • Analysis of locomotion phenotypes in Drosophila larvae with glial gene knockdown.

    • Main Results:

    • FIM provides high resolution and contrast, enabling detection of fluorescent proteins in vivo.
    • FIMTrack significantly increased the number of analyzable locomotion tracks, even in dense populations.
    • Subtle locomotion defects (contraction pattern, head sweeping) were identified in Drosophila larvae lacking kinesin heavy chain (khc) or rab30, which were missed by previous analyses.

    Conclusions:

    • FIM is a versatile imaging technique applicable to various organisms and biological questions.
    • FIM combined with FIMTrack facilitates high-throughput screening of subtle behavioral phenotypes.
    • This approach enables forward genetic screens to uncover the neural underpinnings of behavior.