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Updated: May 14, 2026
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High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay
Published on: March 10, 2020
CrcZ and CrcX regulate carbon source utilization in Pseudomonas syringae pathovar tomato strain DC3000
Melanie J Filiatrault1, Paul V Stodghill, Janet Wilson
1Plant-Microbe Interactions Research Unit, Robert W. Holley Center for Agriculture and Health, Agricultural Research Service, United States Department of Agriculture, Ithaca, NY, USA. melanie.filiatrault@ars.usda.gov
RNA Biology
|January 29, 2013
Summary
Small non-coding RNAs (ncRNAs) like CrcZ and CrcX regulate bacterial virulence. Their expression in Pseudomonas syringae depends on RpoN and CbrAB, varying with carbon sources.
Area of Science:
- Bacteriology
- Molecular Biology
- Genomics
Background:
- Small non-coding RNAs (ncRNAs) are crucial regulators in bacterial pathways, impacting virulence factors.
- Carbon catabolite repression is modulated by small RNAs (crcZ, crcY) in Pseudomonas species.
Purpose of the Study:
- To investigate the regulation of CrcZ and CrcX expression in Pseudomonas syringae pv. tomato DC3000.
- To determine the distribution and subfamily classification of Crc ncRNAs across Pseudomonas genomes.
Main Methods:
- Analysis of crcZ and crcX gene expression.
- Investigating the dependence on RpoN and the CbrAB two-component system.
- Genomic sequence screening of Pseudomonads to identify Crc family members.
Main Results:
- CrcZ and CrcX expression is dependent on RpoN and CbrAB in P. syringae.
- Expression levels are influenced by the available carbon source.
- Pseudomonas genomes harbor variable numbers of Crc ncRNA family members.
- Three main subfamilies (CrcZ, CrcX, CrcY) were identified, with CrcX being unique to P. syringae.
Conclusions:
- RpoN and CbrAB are key regulators of CrcZ and CrcX in P. syringae.
- The Crc ncRNA family shows diversity across Pseudomonas, with a unique CrcX subfamily in P. syringae.
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