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Replication in Prokaryotes

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...

Replication in Prokaryotes

Replication in Prokaryotes

Replication in Prokaryotes

Replication in Prokaryotes

Prokaryotic DNA Replication

Prokaryotic DNA Replication

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...

S-Cdk Initiates DNA Replication

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.

S-Cdk Initiates DNA Replication

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.

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Related Experiment Video

Updated: May 14, 2026

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

Thermococcus kodakarensis DNA replication.

Zhuo Li¹, Lori M Kelman, Zvi Kelman

¹Institute for Bioscience and Biotechnology Research, 9600 Gudelsky Drive, Rockville, MD 20850, USA.

Biochemical Society Transactions

|January 30, 2013

Summary

This summary is machine-generated.

Recent advances in genetic tools have enabled new studies on archaeal DNA replication. Research on Thermococcus kodakarensis provides insights into its replication machinery.

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Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

DNA replication is fundamental to all life.
Archaeal DNA replication research is ~20 years old.
Limited genetic tools previously hindered progress.

Purpose of the Study:

To summarize recent developments in the study of DNA replication machinery.
Focus on the thermophilic euryarchaeon Thermococcus kodakarensis.
Integrate biochemical, structural, and genetic findings.

Main Methods:

Biochemical analyses of replication proteins.
Structural studies of archaeal replication complexes.
Application of genetic approaches in Thermococcus kodakarensis.

Main Results:

Availability of genetic tools for archaeal species, including T. kodakarensis.
Progress in understanding the biochemical and structural aspects of archaeal replication.
Integration of diverse methodologies to study replication.

Conclusions:

Genetic approaches are now feasible for studying archaeal DNA replication.
Thermococcus kodakarensis is a key model organism for these studies.
Recent advancements offer a more comprehensive understanding of archaeal replication machinery.