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Updated: May 14, 2026
DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
Published on: September 27, 2019
Transfer of a protein pattern from self-assembled DNA origami to a functionalized substrate
Katerina Busuttil1, Alexandru Rotaru, Mingdong Dong
1Danish National Research Foundation: Centre for DNA Nanotechnology at Interdisciplinary Nanoscience Center, Aarhus University, Langelandsgade 140, DK-8000 Aarhus C, Denmark.
Abstract:
Patterns of the protein streptavidin on DNA origami are printed onto a functionalized surface. The streptavidins are linked to DNA via a disulfide spacer and these conjugates self-organise into a predetermined pattern on DNA origami. The structure immobilises on a biotin-SAM-functionalized Au surface. By disulfide cleavage the origami is separated from the streptavidins, leaving the protein pattern at the surface.
