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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

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Lensless Fluorescent Microscopy on a Chip
11:23

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Gigapixel fluorescence microscopy with a water immersion microlens array.

Antony Orth1, Kenneth Crozier

  • 1School of Engineering and Applied Sciences, Harvard University, 33 Oxford St., Cambridge, Massachusetts 02138, USA. tonyorth@seas.harvard.edu

Optics Express
|February 8, 2013
PubMed
Summary
This summary is machine-generated.

We developed a high-throughput gigapixel fluorescence microscope using a microlens array. This system images 384-well micro plates at high resolution and speed, significantly improving on current technology.

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Area of Science:

  • Biophotonics
  • Microscopy
  • High-throughput screening

Background:

  • Microscopy is crucial for biological research, but high-throughput imaging of large sample areas remains a challenge.
  • Existing systems often lack the speed and resolution required for comprehensive analysis of micro well plate formats.

Purpose of the Study:

  • To demonstrate a novel high-throughput gigapixel fluorescence microscopy system.
  • To enable rapid, high-resolution imaging of samples within micro well plates.

Main Methods:

  • Utilized a microlens array in a parallelized microscopy setup.
  • Developed a system capable of imaging centimeter-scale regions of 384-well micro well plates.
  • Achieved 1.72 μm resolution and a raw pixel throughput of 25.4 Mpx/s.

Main Results:

  • Demonstrated imaging of 384-well micro well plates, a first for parallelized microscopy systems.
  • Achieved a sample pixel throughput of 13.2 Mpx/s, more than double the commercial state-of-the-art.
  • Successfully performed fluorescent imaging of tissue samples through coverslips.

Conclusions:

  • The developed gigapixel fluorescence microscope offers unprecedented speed and resolution for micro well plate imaging.
  • This technology has significant potential for applications in drug discovery, diagnostics, and fundamental biological research.