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Related Concept Videos

Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Mismatch Repair01:36

Mismatch Repair

Overview

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Related Experiment Video

Updated: May 14, 2026

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

DNA repair: trust but verify.

Maria Spies1

  • 1Department of Biochemistry, University of Iowa Carver College of Medicine, IA 52242, USA. maria-spies@uiowa.edu

Current Biology : CB
|February 9, 2013
PubMed
Summary

This study clarifies the role of XPD helicase in DNA repair. It confirms XPD helicase verifies DNA damage for the nucleotide excision repair pathway.

Area of Science:

  • Molecular Biology
  • DNA Repair Mechanisms
  • Biochemistry

Background:

  • DNA damage recognition is crucial for maintaining genomic stability.
  • The nucleotide excision repair (NER) pathway is a major DNA repair mechanism.
  • The precise function of XPD helicase in NER has been debated.

Purpose of the Study:

  • To determine if XPD helicase verifies DNA damage suitability for NER.
  • To resolve the ongoing debate regarding XPD helicase's role in damage verification.

Main Methods:

  • The study likely involved in vitro biochemical assays.
  • Analysis of protein-DNA interactions and enzymatic activity.
  • Potentially, cellular assays to observe NER pathway function.

More Related Videos

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Related Experiment Videos

Last Updated: May 14, 2026

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Main Results:

  • Evidence suggests XPD helicase plays a verification role.
  • Findings indicate XPD helicase assesses damage before NER initiation.
  • The study provides a definitive answer to the verification question.

Conclusions:

  • XPD helicase actively verifies DNA damage for NER.
  • This verification step is essential for NER pathway fidelity.
  • The findings enhance our understanding of DNA repair regulation.