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Updated: May 14, 2026

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
Published on: October 15, 2019
Directly from the source: endogenous preparations of molecular machines
Pablo Mesa1, Aurélien Deniaud, Guillermo Montoya
1Structural Biology and Biocomputing Programme, Spanish National Cancer Research Centre, Macromolecular Crystallography Group, c/Melchor Fdez. Almagro 3, 28029 Madrid, Spain.
Abstract:
Purification from a source enriched in large macromolecular machines with basic cellular function is still the method of choice in many cases. Such complexes occur in sufficiently high copy numbers in the cell and can be isolated using classical protein purification protocols. Although advanced DNA recombinant technologies and sophisticated overexpression strategies are available, many complexes like the ribosome, RNA polymerase II and membrane protein complexes involved in photosynthesis or in oxidative phosphorylation can only be purified from a rich source. Here, we review recent accomplishments and limitations in applying this strategy.
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