Related Experiment Video
Updated: May 14, 2026

Bioprospecting of Extremophilic Microorganisms to Address Environmental Pollution
Published on: December 30, 2021
Applied development of crude enzyme from Bacillus cereus in prebiotics and microbial community changes in soil
Tzu-Wen Liang1, Chin-Pei Liu, Chunhung Wu
1Life Science Development Center, Tamkang University, New Taipei City 25137, Taiwan.
Abstract:
The chitosanase and chitinase activity were revealed in the culture supernatant of Bacillus cereus TKU027 with shrimp head powder (SHP) as the sole carbon/nitrogen source. The chitosan with 60% degree of deacetylation (DD) was depolymerized by TKU027 crude enzyme. The low DP oligomers stimulated the growth of Lactobacillus paracasei BCRC12193 and Lactobacillus kefir BCRC14011 in a MRS broth supplemented with low DP oligomers for 12 h. Conversely, the high DP oligomers (0.1%) had potent inhibitory effects against L. paracasei BCRC12193 and L. kefir BCRC14011 for 48 h. Besides, the study also investigated the effects of B. cereus TKU027 on degradation of SHP and the survival conditions of bacteria in mangrove river sediment of Tamsui River. The 5 weeks-incubation sample of SHP and B. cereus TKU027-amended mangrove river sediment showed the highest amounts of biomass, reducing sugar and total sugar, and some variance of bacterial community compositions existed in the soils.
Related Concept Videos
Environmental Applications of Microorganisms
Microbes in the Production of Fermented Foods
iChip
Production of Biopesticides
Microorganisms in Agriculture and Food industry
Bioreactor Controls-III
