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Preparation and Friction Force Microscopy Measurements of Immiscible, Opposing Polymer Brushes

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Published on: December 24, 2014

Protein-resistant hyperbranched polyethyleneimine brush surfaces.

Subramanian Suriyanarayanan¹, Hung-Hsun Lee, Bo Liedberg

¹Bioorganic & Biophysical Chemistry Laboratory, Linnæus University Centre for Biomaterials Chemistry, Linnæus University, Kalmar, Sweden.

Journal of Colloid and Interface Science

|February 14, 2013

Summary

This summary is machine-generated.

A new polyethyleneimine (PEI) surface effectively resists protein adsorption, showing promise for biomedical applications. Its protein resistance improves with ionic strength and remains stable over time.

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Area of Science:

Materials Science
Surface Chemistry
Biomaterials Engineering

Background:

Non-specific protein adsorption onto surfaces is a significant challenge in biomedical devices and biosensors.
Developing robust, protein-resistant surfaces is crucial for improving device performance and longevity.
Polyethyleneimine (PEI) offers potential as a surface modification agent due to its versatile chemistry.

Purpose of the Study:

To design and fabricate a novel hyperbranched polyethyleneimine (PEI) modified gold surface.
To investigate the protein-resistant properties of this PEI-modified surface.
To evaluate the stability and performance of the PEI brushes under various conditions.

Main Methods:

Fabrication of PEI brushes on gold surfaces via self-assembly using 11-mercaptoundecanoic acid (MuDA) monolayers and carbodiimide chemistry.
Characterization using X-ray photoelectron spectroscopy (XPS), reflection absorption infrared spectroscopy (RAIRS), and ellipsometry.
Evaluation of protein adsorption using quartz crystal microbalance (QCM) under flow injection analysis (FIA) conditions.

Main Results:

PEI brush thickness and surface concentration increased with adsorption solution ionic strength.
Protein adsorption (cytochrome C, BSA, ribonuclease A) was suppressed to <0.08μg/cm(2).
Protein resistance improved with higher ionic strength and demonstrated exceptional stability for 6 months.

Conclusions:

Hyperbranched PEI-modified gold surfaces exhibit excellent protein resistance, comparable to PEG-coated surfaces.
The protein-repelling performance is tunable by adjusting the ionic strength of the carrier solution.
These PEI brushes represent a stable and promising alternative for creating protein-resistant surfaces in various applications.