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Published on: February 9, 2019
Erythrocyte membrane-cloaked polymeric nanoparticles for controlled drug loading and release
Santosh Aryal1, Che-Ming J Hu, Ronnie H Fang
1Department of Nano Engineering, University of California, San Diego, 9500 Gilman Drive, MC-0448, La Jolla, CA 92093-0448, USA.
Aim:
Polymeric nanoparticles (NPs) cloaked by red blood cell membrane (RBCm) confer the combined advantage of both long circulation lifetime and controlled drug release. The authors carried out studies to gain a better understanding of the drug loading, drug-release kinetics and cell-based efficacy of RBCm-cloaked NPs.
Materials & Methods:
Two strategies for loading doxorubicin into the RBCm-cloaked NPs were compared: physical encapsulation and chemical conjugation. In vitro efficacy was examined using the acute myeloid leukemia cell line, Kasumi-1.
Results:
It was found that the chemical conjugation strategy resulted in a more sustained drug release profile, and that the RBCm cloak provided a barrier, retarding the outward diffusion of encapsulated drug molecules. It was also demonstrated that RBCm-cloaked NPs exhibit higher toxicity in comparison with free doxorubicin.
Conclusion:
These results indicate that the RBCm-cloaked NPs hold great promise to become a valuable drug-delivery platform for the treatment of various diseases such as blood cancers.
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