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Updated: May 14, 2026

Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly
Published on: April 25, 2016
The history and advances of reversible terminators used in new generations of sequencing technology
Fei Chen1, Mengxing Dong, Meng Ge
1CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China. chenfei@big.ac.cn
Abstract:
DNA sequencing using reversible terminators, as one sequencing by synthesis strategy, has garnered a great deal of interest due to its popular application in the second-generation high-throughput DNA sequencing technology. In this review, we provided its history of development, classification, and working mechanism of this technology. We also outlined the screening strategies for DNA polymerases to accommodate the reversible terminators as substrates during polymerization; particularly, we introduced the "REAP" method developed by us. At the end of this review, we discussed current limitations of this approach and provided potential solutions to extend its application.
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