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Related Concept Videos

PCR01:32

PCR

Overview
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

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Related Experiment Video

Updated: May 14, 2026

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems
07:35

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

Published on: June 14, 2021

Isothermal amplification system based on template-dependent extension.

Hui Zhou1, Su-Jin Xie, Song-Bai Zhang

  • 1State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.

Chemical Communications (Cambridge, England)
|February 19, 2013
PubMed
Summary
This summary is machine-generated.

A new template-dependent extension based isothermal amplification (TEIA) system offers high single-base discrimination. This method significantly reduces non-specific reactions in isothermal strand displacement amplification (SDA) for improved detection devices.

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Last Updated: May 14, 2026

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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Isothermal strand displacement amplification (SDA) is a nucleic acid amplification technique.
  • Non-specific reactions can interfere with the accuracy of SDA-based detection.
  • There is a need for improved methods to enhance specificity in isothermal amplification.

Purpose of the Study:

  • To develop a novel isothermal amplification system with enhanced specificity.
  • To improve single-base discrimination capabilities in nucleic acid detection.
  • To overcome limitations of non-specific reactions in existing isothermal amplification techniques.

Main Methods:

  • Development of a template-dependent extension based isothermal amplification (TEIA) system.
  • Utilizing a functionalized template probe to guide the amplification process.
  • Comparative analysis with standard isothermal strand displacement amplification (SDA) to evaluate specificity.

Main Results:

  • The developed TEIA system demonstrated high single-base discrimination capability.
  • Substantial avoidance of interference from non-specific reactions was achieved.
  • The functionalized template probe effectively improved the specificity of the amplification.

Conclusions:

  • The novel TEIA system offers a significant advancement over traditional SDA.
  • This technology shows high potential for developing more accurate and reliable SDA-based detection devices.
  • The strategy of using functionalized template probes is effective for enhancing specificity in isothermal amplification.