Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Glassware Calibration01:11

Glassware Calibration

Accurate calibration of glassware, such as volumetric flasks, pipettes, and burettes, is essential to ensure accurate measurements in the analytical laboratory. Calibration helps maintain consistency across measurements and prevents errors arising from inaccurate volumes.
Volumetric flasks: Volumetric flasks are designed to prepare aqueous solutions of precise volumes accurately with a calibration line on the neck. To calibrate a volumetric flask, it is important to fill it with distilled...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nontoxic In Vivo Clearable Nanoparticle Clusters for Theranostic Applications.

ACS biomaterials science & engineering·2022
Same author

Biodegradable Protein-Stabilized Inorganic Nanoassemblies for Photothermal Radiotherapy of Hepatoma Cells.

ACS omega·2022
Same author

Effect of Preoperative Continuation of Aspirin on Postoperative Bleeding After Off-Pump Coronary Artery Bypass Graft: A Prospective Cohort Study.

Cureus·2021
Same author

Quantitative estimation of mechanical and optical properties from ultrasound assisted optical tomography data.

Journal of biomedical optics·2012
Same author

Practical fully three-dimensional reconstruction algorithms for diffuse optical tomography.

Journal of the Optical Society of America. A, Optics, image science, and vision·2012
Same author

Ultrasound-modulated optical tomography: recovery of amplitude of vibration in the insonified region from boundary measurement of light correlation.

Journal of the Optical Society of America. A, Optics, image science, and vision·2011
Same journal

Segmentation-guided photon pooling enables robust single-cell analysis and fast fluorescence lifetime imaging microscopy.

Journal of biomedical optics·2026
Same journal

Method of spatial scanning of modulated laser radiation for outline imaging of interphalangeal joints.

Journal of biomedical optics·2026
Same journal

Multimodal optical imaging for the assessment of the teratogenic effects of ethanol on zebrafish development.

Journal of biomedical optics·2026
Same journal

Fluorescence properties of collagen types I-V: a comprehensive study of spectral and lifetime characteristics.

Journal of biomedical optics·2026
Same journal

Spectral dependence of lipofuscin fluorescence lifetimes revealed by FLIM with a superconducting nanowire single-photon detector.

Journal of biomedical optics·2026
Same journal

Building the future of biophotonics through experiential education and seasonal schools.

Journal of biomedical optics·2026
See all related articles

Related Experiment Video

Updated: May 14, 2026

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
12:24

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

Flux density calibration in diffuse optical tomographic systems.

Samir Kumar Biswas1, Kanhirodan Rajan, Ram M Vasu

  • 1Indian Institute of Science, Department of Physics, Bangalore, India.

Journal of Biomedical Optics
|February 21, 2013
PubMed
Summary
This summary is machine-generated.

Two methods improve diffuse optical tomography (DOT) by correcting system transfer functions. These techniques accurately estimate true flux density from measured data, enhancing light transport modeling in scattering tissues.

More Related Videos

Determining 3D Flow Fields via Multi-camera Light Field Imaging
14:25

Determining 3D Flow Fields via Multi-camera Light Field Imaging

Published on: March 6, 2013

Fiber Optic Distributed Sensors for High-resolution Temperature Field Mapping
09:48

Fiber Optic Distributed Sensors for High-resolution Temperature Field Mapping

Published on: November 7, 2016

Related Experiment Videos

Last Updated: May 14, 2026

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
12:24

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

Determining 3D Flow Fields via Multi-camera Light Field Imaging
14:25

Determining 3D Flow Fields via Multi-camera Light Field Imaging

Published on: March 6, 2013

Fiber Optic Distributed Sensors for High-resolution Temperature Field Mapping
09:48

Fiber Optic Distributed Sensors for High-resolution Temperature Field Mapping

Published on: November 7, 2016

Area of Science:

  • Biomedical Optics
  • Medical Imaging
  • Photonics

Background:

  • Diffuse optical tomography (DOT) models light transport in scattering tissues using the finite element method, yielding flux density (Φ) at mesh nodes.
  • Experimentally measured flux (Umeasured) requires correction for system transfer functions (R) to accurately represent true flux density.

Purpose of the Study:

  • To present and validate two novel methods for compensating system transfer function perturbations in DOT.
  • To accurately estimate true flux density (Φ) from experimentally measured flux (Umeasured) in diffuse optical tomography.

Main Methods:

  • Method 1: Calibrates the DOT system using measurement data from a homogeneous phantom (Umeasuredhomo).
  • Method 2: Estimates homogeneous phantom measurements from heterogeneous phantom data (Umeasuredhetero) alone, eliminating the need for a separate homogeneous phantom.
  • Statistical averaging and redistribution of heterogeneous phantom data to detector positions are employed in the second method.

Main Results:

  • Both presented methods effectively compensate for system transfer function perturbations.
  • The second method successfully estimates true flux density without requiring a homogeneous phantom.
  • Experiments on various phantoms (tissue-mimicking, pork) and a human hand demonstrate the robustness of the developed approaches.

Conclusions:

  • The proposed methods offer robust solutions for correcting measurement system effects in diffuse optical tomography.
  • The second method provides a more efficient approach by obviating the need for homogeneous phantom measurements.
  • Accurate flux density estimation is crucial for reliable light transport modeling in biomedical applications of DOT.