Single and combined effects of estrone and 17β-estradiol on male goldfish
Zhen Hua Yan1, Guang Hua Lu, Xiao Fan Yang
1Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, Jiangsu, China.
Objective:
To assess the single and combined effects of estrone (E1) and 17β-estradiol (E2) on goldfish (Carassius auratus).
Methods:
Batch tests were conducted. Serum levels of vitellogenin (VTG) and E2, gonadosomatic indices (GSI), gonadal DNA damage and liver 7-ethoxyresorufin-O-deethylase (EROD) activity were measured after exposure for 14 days.
Results:
The VTG level increased significantly in a concentration-dependent manner. The serum E2 level was significantly higher and the GSI level was significantly lower in goldfish after exposed to the 3 drugs. DNA damage occurred in treated samples and EROD activity was significantly suppressed 7 days after exposure. The joint effect of E1 and E2 was additive with regard to VTG induction.
Conclusion:
The results of our study highlight a series of effects of steroidal estrogens on goldfish. Further study is needed to confirm their effect as a whole.
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