Related Experiment Video
Updated: May 14, 2026

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology
Published on: March 12, 2015
Vinyl sulfone-based ferrocenylation reagents: applications in conjugation and bioconjugation
Alicia Megia-Fernandez1, Fernando Hernandez-Mateo, Francisco Santoyo-Gonzalez
1Departamento de Q. Orgánica, Facultad de Ciencia, Instituto de Biotecnologia, Universidad de Granada, Granada, 18071 Spain.
Abstract:
The easy vinyl sulfone derivatization of ferrocene allows the preparation of some effective, versatile and valuable ferrocenylation reagents. The applicability of such compounds in conjugation and bioconjugation of amine and/or thiol containing molecules and biomolecules through Michael-type addition under mild conditions that preserve the biological function of the latter is described. The feasibility of the methodology is demonstrated by the preparation of a variety of conjugates and bioconjugates (ferrocenyl terminated dendrimers and ferrocene-sugar, ferrocene-cyclodextrin, ferrocene-peptide and ferrocene-protein conjugates).
Related Concept Videos
Phase II Reactions: Sulfation and Conjugation with α-Amino Acids
Phase II Conjugation Reactions: Overview
Drug Metabolism: Phase II Reactions
Electrophilic Aromatic Substitution: Sulfonation of Benzene
Limitations of Friedel–Crafts Reactions
Phase II Reactions: Miscellaneous Conjugation Reactions
A key example involves the conjugation of cyanide ions, which impair cellular respiration and alter hemoglobin into non-oxygen-carrying cyanmethemoglobin. To neutralize this threat, a sulfur atom from thiosulphate is transferred to the cyanide ion, catalyzed by the enzyme rhodanese, resulting in an inactive compound called thiocyanate. The production of...

