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Updated: May 13, 2026

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Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC)
Published on: March 15, 2013
Intracellular activation of acetyl-CoA by an artificial reaction promoter and its fluorescent detection
Hirokazu Komatsu1, Yutaka Shindo, Shigehiro A Kawashima
1Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Abstract:
The application of a new rhodamine-based fluorescent probe, RH-NH2 3 and an acyl transfer promoter, PBu3, to Hela cells induced a time-dependent increase in fluorescence in the mitochondria, which was most likely due to acetylation of RH-NH2 3 with activated acetyl-CoA by the artificial reaction promoter in living cells.

