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Raman Spectroscopy Instrumentation: Overview

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

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Updated: May 13, 2026

Intradermal Microdialysis: An Approach to Investigating Novel Mechanisms of Microvascular Dysfunction in Humans

Intradermal Microdialysis: An Approach to Investigating Novel Mechanisms of Microvascular Dysfunction in Humans

Published on: July 21, 2023

A subcutaneous Raman needle probe.

John C C Day¹, Nicholas Stone

¹Interface Analysis Centre, University of Bristol, Bristol BS2 8BS, UK. j.c.c.day@bris.ac.uk

Applied Spectroscopy

|March 5, 2013

Summary

This summary is machine-generated.

Researchers developed a miniature fiber optic probe for optical biopsies using Raman spectroscopy. This technology aids in diagnosing diseases and identifying critical tissue sites for medical procedures.

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Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

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Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

Area of Science:

Biomedical Optics
Spectroscopy
Medical Device Engineering

Background:

Raman spectroscopy offers detailed biochemical analysis of tissues and cells.
Current methods for tissue analysis can be invasive or lack real-time biochemical information.
Minimally invasive diagnostic tools are crucial for early disease detection and precise medical interventions.

Purpose of the Study:

To design and construct a miniature fiber optic probe for Raman spectroscopy.
To integrate this probe into a standard hypodermic needle for optical biopsies.
To assess the feasibility of using this device for in vivo tissue analysis.

Main Methods:

Development of a novel optical design for a miniaturized fiber optic probe.
Fabrication of the probe for integration with hypodermic needles.
Testing with ex vivo tissue samples to obtain Raman spectra.

Main Results:

Successful design and fabrication of a miniature fiber optic Raman probe.
Demonstration of the probe's capability to acquire spectra from various ex vivo tissues.
Initial data suggests potential for differentiating tissue types and disease states.

Conclusions:

The developed fiber optic Raman probe is feasible for optical biopsies.
This technology can provide valuable biochemical information from solid tissues.
Potential applications include disease diagnosis and identification of critical anatomical sites.