Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Morphological artifacts in pulmonary pathology and literature review.

Virchows Archiv : an international journal of pathology·2026
Same author

Hybrid particle-wave Monte Carlo OCT simulation method provides a three orders of magnitude improvement in efficiency.

Biomedical optics express·2026
Same author

A Learning Accelerator Framework: Scalable Clinical Artificial Intelligence Development and Delivery.

Journal of the American College of Radiology : JACR·2025
Same author

Comparison of Event-based Analysis Versus Trend-based Analysis in the Detection of Glaucoma Progression by Optical Coherence Tomography 3-Dimensional Rim Measurements.

Journal of glaucoma·2025
Same author

Chromatic dispersion based axial length estimation using retinal spectral domain optical coherence tomography.

Biomedical optics express·2025
Same author

Class of nonlinear filtering and windowing methods for image processing and reconstruction.

Optics letters·2025
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: May 13, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Three-dimensional intracellular optical coherence phase imaging.

Frank Helderman1, Bryan Haslam, Johannes F de Boer

  • 1Department of Physics and Astronomy, VU University Amsterdam, De Boelelaan 1081, Amsterdam 1081 HV, The Netherlands.

Optics Letters
|March 5, 2013
PubMed
Summary
This summary is machine-generated.

This study introduces a dual-beam optical coherence phase microscopy (OCPM) setup. It achieves high-resolution, 3D intracellular imaging by overcoming depth-of-field limitations for precise nanoscale measurements.

More Related Videos

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Related Experiment Videos

Last Updated: May 13, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Area of Science:

  • Biomedical Optics
  • Cellular Imaging
  • Nanoscale Microscopy

Background:

  • Quantitative phase imaging enables label-free studies of cellular nanoscale structure and dynamics.
  • Optical coherence phase microscopy (OCPM) offers high sensitivity for quantitative phase information.
  • Traditional OCPM faces depth-of-field limitations with high numerical aperture objectives, hindering intracellular analysis.

Purpose of the Study:

  • To develop an improved OCPM system for high-resolution, 3D intracellular phase imaging.
  • To overcome the trade-off between resolution and depth of field in OCPM.
  • To enhance phase stability for accurate nanoscale measurements within cells.

Main Methods:

  • Designed a novel dual-beam sample arm for OCPM.
  • Utilized a large-diameter beam for high-resolution imaging with a 1.2 N.A. objective.
  • Employed a small-diameter beam with a larger depth of field for stable reference phase detection from the cover glass.

Main Results:

  • Achieved phase stability with a standard deviation of 0.021 rad (0.9 nm optical path displacement).
  • Determined lateral and axial point spread function dimensions of 0.42 μm and 0.84 μm, respectively.
  • Demonstrated the system's capability for detailed 3D intracellular phase imaging.

Conclusions:

  • The dual-beam OCPM setup effectively addresses depth-of-field limitations for high-resolution imaging.
  • This approach enables sensitive, quantitative phase measurements within cells.
  • The system is ideal for advanced 3D intracellular nanoscale studies.