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Updated: May 13, 2026
Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells
Published on: March 8, 2019
Expression profiles of subtracted mRNAs during cellular senescence in human mesenchymal stem cells derived from bone
Jung Ki Yoo1, Seong-jun Choi, Jin Kyeoung Kim
1Department of Pharmacy, College of Pharmacy, CHA University, 222 Yatap-dong, Bundang-gu, Seongnam-si, Gyeonggi-do 463-836, Republic of Korea.
Abstract:
Cellular senescence is an irreversible cell cycle arrest that limits the replicative lifespan of cells. Senescence suppresses development of tumors by regulating aging factors, such as cyclin dependent kinase inhibitor (CKI) and telomerase. Suppression subtractive hybridization (SSH) was used to identify genes that were differentially expressed between young human mesenchymal stem cells (Y-hMSCs) and senescent human mesenchymal stem cells (S-hMSCs). We selected positive clones that were functionally characterized by referring to public databases using NCBI BLAST tool. This search revealed that 19 genes were downregulated, and 43 genes were upregulated in S-hMSCs relative to Y-hMSCs. Among subtracted clones in Y-hMSCs, most of genes markedly were related to metabolic functions. These genes, PDIA3, WDR1, FSTL1, COPG1, LMAN1, and PDIA6, significantly downregulated. Conversely, genes for subtracted clones in S-hMSCs were mostly associated with cell adhesion. In particular, the expression levels of 9 genes, HSP90B1, EID1, ATP2B4, DDAH1, PRNP, RAB1A, PGS5, TM4SF1 and SSR3, gradually increased during senescence. These genes have not previously been identified as being related to cellular senescence, but they seemed to be potentially affected during cellular senescence.
Insights
Cellular senescence, an irreversible cell cycle arrest, involves changes in gene expression. This study identified novel genes potentially affected during senescence in human mesenchymal stem cells.
Area of Science:
- Cell Biology
- Molecular Biology
- Genomics
Background:
- Cellular senescence is a state of irreversible cell cycle arrest.
- Senescence plays a role in tumor suppression by regulating aging factors.
- Understanding gene expression changes in senescence is crucial for aging research.
Purpose of the Study:
- To identify genes differentially expressed between young and senescent human mesenchymal stem cells (hMSCs).
- To uncover novel genes potentially involved in the cellular senescence process.
Main Methods:
- Suppression subtractive hybridization (SSH) was employed to compare gene expression profiles.
- Differential gene expression was analyzed between young hMSCs (Y-hMSCs) and senescent hMSCs (S-hMSCs).
- Gene functions were characterized using NCBI BLAST and public databases.
Main Results:
- 19 genes were downregulated and 43 genes were upregulated in S-hMSCs compared to Y-hMSCs.
- Downregulated genes in Y-hMSCs were primarily associated with metabolic functions (e.g., PDIA3, WDR1).
- Upregulated genes in S-hMSCs were predominantly linked to cell adhesion, with 9 novel genes identified (e.g., HSP90B1, PRNP).
Conclusions:
- Cellular senescence is characterized by significant alterations in gene expression profiles.
- Metabolic and cell adhesion pathways are notably affected during senescence.
- Several newly identified genes may play a role in cellular senescence and warrant further investigation.
Related Concept Videos
02:15Replicative Cell Senescence
02:15Replicative Cell Senescence
01:19Mesenchymal Stem Cells