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Related Experiment Video

Updated: May 13, 2026

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans
11:26

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Published on: February 7, 2018

Highlighted Ca²⁺ imaging with a genetically encoded 'caged' indicator.

Tomoki Matsuda1, Kazuki Horikawa, Kenta Saito

  • 1The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.

Scientific Reports
|March 12, 2013
PubMed
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Researchers developed a photoactivatable caged calcium (Ca2+) indicator, PA-TNXL, enabling controlled visualization of cellular activity. This novel tool allows precise imaging of Ca2+ dynamics in specific cells and compartments for advanced bioimaging research.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Bioimaging

Background:

  • Genetically encoded fluorescent indicators are vital for visualizing biological processes.
  • Current indicators lack control over expression timing and location.
  • A need exists for single-cell visualization techniques to study cellular heterogeneity.

Purpose of the Study:

  • To develop a controllable, genetically encoded indicator for calcium (Ca2+) dynamics.
  • To overcome limitations of existing bioimaging tools regarding specificity and control.
  • To enable visualization of Ca2+ in specific cells or cellular compartments.

Main Methods:

  • Development of PA-TNXL, a photoactivatable caged Ca2+ indicator.
  • Utilizing a Förster Resonance Energy Transfer (FRET) system with a photoactivatable donor (PA-GFP) and a quencher.

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Last Updated: May 13, 2026

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Published on: February 7, 2018

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  • Activation of the indicator via ultraviolet irradiation for controlled imaging.

    • Main Results:

    • Successful imaging of Ca2+ dynamics in HeLa cells and hippocampal neurons.
    • Demonstration of controlled expression timing and location of the indicator.
    • Validation of PA-TNXL as a tool for specific cellular Ca2+ visualization.

    Conclusions:

    • PA-TNXL provides precise spatiotemporal control over Ca2+ indicator expression.
    • This technology advances the study of cellular individuality and dynamics.
    • The developed platform facilitates the creation of novel photoactivatable indicators for future research.