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Updated: May 13, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
Comparative metabolite profiling and chemical study of Ramalina siliquosa complex using LC-ESI-MS/MS approach
Delphine Parrot1, Saleem Jan, Nicolas Baert
1Equipe PNSCM Produits naturels - Synthèses - Chimie Médicinale, UMR CNRS 6226, Institut des Sciences chimiques de Rennes, UFR Sciences Pharmaceutiques et Biologiques, Univ. Rennes 1, Université Européenne de Bretagne, 2 Avenue du Pr. Léon Bernard, F-35043 Rennes, France.
Abstract:
A chemical study of the lichen Ramalina siliquosa complex found in Brittany was conducted. Eight chemotypes were considered and their chemical composition was elucidated for the first time by LC-MS analysis. Ten main compounds were identified: conhypoprotocetraric acid (1), salazinic acid (2), peristictic acid (3), cryptostictic acid (4), protocetraric acid (5), stictic acid (6), norstictic acid (7), hypoprotocetraric acid (8), 4-O-demethylbarbatic acid (9), (+)-usnic acid (10) and 22 minor compounds were reported. The MS/MS fragmentation patterns of each compound of R. siliquosa complex were determined and proposed.
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