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Updated: May 13, 2026
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Urethral Stricture Induction Followed by Buccal Mucosa Graft Urethroplasty in a Rat Model
Published on: April 28, 2023
Urethral reconstruction with tissue engineering and RNA interference techniques in rabbits.
Chao Li1, Yue-Min Xu, Zhang-Shun Liu
1Department of Urology, Affiliated Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, People's Republic of China. chaoli1979@126.com
Urology
|March 16, 2013
Summary
Oral keratinocytes and TGF-β1 siRNA transfected fibroblasts show promise for urethral tissue engineering. This approach successfully reconstructed urethras in rabbits, preventing strictures and promoting tissue regeneration.
Area of Science:
- Regenerative Medicine
- Urology
- Biomaterials Science
Background:
- Urethral reconstruction presents challenges due to limited cell sources.
- Tissue engineering offers a promising alternative for urethral repair.
Purpose of the Study:
- To evaluate the feasibility of using oral keratinocytes and TGF-β1 siRNA transfected fibroblasts on BAMGs for urethral reconstruction.
- To assess the efficacy of this tissue-engineered construct in a rabbit model.
Main Methods:
- Oral keratinocytes and TGF-β1 siRNA transfected fibroblasts were seeded onto bladder acellular matrix grafts (BAMGs).
- Tissue-engineered mucosa was characterized in vitro.
- Urethroplasty was performed in rabbits using cell-seeded BAMGs or acellular BAMGs.
- Urethral reconstruction was evaluated via urethrography and histological analysis.
Main Results:
- Oral keratinocytes and TGF-β1 siRNA transfected fibroblasts demonstrated good biocompatibility with BAMGs.
- BAMGs seeded with both cell types (group 1) and oral keratinocytes alone (group 2) maintained urethral caliber and showed epithelial regeneration.
- Acceullar BAMGs (group 3) resulted in urethral strictures, fibrosis, and inflammation.
- Group 1 exhibited superior results with stratified epithelial layer and capillary formation after 6 months.
Conclusions:
- Oral keratinocytes and TGF-β1 siRNA transfected fibroblasts are suitable seed cells for urethral tissue engineering.
- This combination facilitates successful urethral reconstruction with improved tissue regeneration and vascularization.
