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Related Experiment Video

Updated: May 13, 2026

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Mouse Naïve CD4⁺ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

IL-27 paves different roads to Tr1.

Ajithkumar Vasanthakumar¹, Axel Kallies

¹Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.

European Journal of Immunology

|March 19, 2013

Summary

Interleukin-27 (IL-27) induces IL-10 production in Tr1 cells via STAT3 and Egr-2, independent of c-Maf/Ahr. This reveals plasticity in CD4(+) T cell differentiation pathways.

Area of Science:

Immunology
Cell Biology
Molecular Biology

Background:

Tr1 cells are regulatory CD4(+) T cells that secrete IL-10.
Interleukin-27 (IL-27) promotes Tr1 cell differentiation and IL-10 production, partly via c-Maf and Ahr transcription factors.

Purpose of the Study:

To investigate an alternative IL-27-regulated transcriptional network for IL-10 induction in Tr1 cells.
To elucidate the molecular mechanisms underlying IL-10 production by Tr1 cells.

Main Methods:

Analysis of transcription factor activation (STAT3, Egr-2, c-Maf, Ahr, Blimp1).
Investigation of IL-10 gene induction in Tr1 cells.
Study of IL-27 signaling pathways.

Main Results:

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IL-27 activates STAT3 and Egr-2 transcription factors in a Blimp1-dependent manner to induce IL-10.

This STAT3/Egr-2 pathway is independent of c-Maf and Ahr.

IL-10 induction in Tr1 cells is context-dependent, influenced by cytokine environment and molecular factors.

Conclusions:

IL-27 utilizes a distinct transcriptional network involving STAT3 and Egr-2 for IL-10 induction in Tr1 cells.
Tr1 cells exhibit plasticity, potentially representing a developmental endpoint rather than a distinct lineage.
Findings highlight the complex regulation of T helper cell differentiation and function.