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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: May 13, 2026

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Published on: November 29, 2018

Determining target engagement in living systems

Gabriel M Simon¹, Micah J Niphakis, Benjamin F Cravatt

¹Abide Therapeutics, San Diego, California, USA.

Nature Chemical Biology

|March 20, 2013

Summary

No abstract available in PubMed .

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