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Studying the Effects of Temperature on the Nucleation and Growth of Nanoparticles by Liquid-Cell Transmission Electron Microscopy
Published on: February 17, 2021
Slight temperature changes affect protein affinity and cellular uptake/toxicity of nanoparticles
Morteza Mahmoudi1, Mohammad A Shokrgozar, Shahed Behzadi
1Department of Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Mahmoudi-m@tums.ac.ir
Nanoscale
|March 20, 2013
Summary
Body temperature fluctuations significantly alter nanoparticle protein corona composition and cell interactions. This finding allows for optimized nanoparticle delivery and reduced toxicity by adjusting temperature.
Area of Science:
- Nanotechnology
- Biomedical Engineering
- Cell Biology
Background:
- Nanoparticles (NPs) interact with biological systems via a protein corona.
- NP protein corona composition is known to depend on NP properties.
- The influence of temperature variations on protein corona formation and NP cellular interactions has been overlooked.
Purpose of the Study:
- To investigate the effect of temperature on protein corona composition.
- To determine how temperature influences protein affinity to NP surfaces.
- To examine subsequent cellular responses to temperature-modulated protein-coated NPs.
Main Methods:
- Characterization of protein corona composition under varying temperatures.
- Assessment of protein-NP surface affinity at different temperatures.
- Evaluation of cellular uptake, dispersion, and toxicity of NPs exposed to temperature changes.
Main Results:
- Temperature variations significantly alter protein corona composition and protein-NP affinity.
- Cellular entrance, dispersion, and toxicity of NPs are demonstrably affected by slight temperature changes.
- These findings highlight temperature as a critical factor in NP-cell interactions.
Conclusions:
- Temperature is a crucial, yet previously ignored, factor influencing nanoparticle behavior in biological systems.
- Understanding temperature-dependent protein corona formation can guide the design of NPs for targeted delivery.
- Modulating cellular or organ temperature may offer a strategy to enhance NP efficacy and reduce toxicity.
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