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Updated: May 13, 2026

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A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
Published on: September 10, 2014
Direct electrical single-molecule detection of DNA through electron transfer induced by hybridization
Tomoaki Nishino1, Phuc Tan Bui
1Nanoscience and Nanotechnology Research Center, Osaka Prefecture University, Sakai, Osaka 599-8570, Japan. t-nishino@21c.osakafu-u.ac.jp
Abstract:
Single-stranded DNA was utilized as a probe tip for single-molecule DNA detection. Hybridization of the DNA tip and target DNA induces electron tunneling through the resulting DNA duplex. It is demonstrated that the DNA tip allows not only genetic detection but also discovery of single-nucleotide polymorphisms at the single-molecule level.

