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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Related Experiment Video

Updated: Apr 17, 2026

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization
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Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization

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Cell-SELEX Technology.

Shoji Ohuchi1

  • 1Department of Basic Medical Sciences, Institute of Medical Science, University of Tokyo , Tokyo, Japan .

Bioresearch Open Access
|March 22, 2013
PubMed
Summary
This summary is machine-generated.

Cell-SELEX technology uses live cells for aptamer selection, offering functional aptamers against native targets. This method enables aptamer generation without prior knowledge of cell surface molecules.

Keywords:
biomarkerscancer researchnucleic acidsstem cells

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biochemistry

Background:

  • Aptamers are nucleic acid ligands with high target affinity, serving as alternatives to antibodies in various applications.
  • Systematic Evolution of Ligands by Exponential Enrichment (SELEX) is the standard method for aptamer identification.
  • Traditional SELEX often uses purified targets, which can be challenging for certain molecules like transmembrane proteins.

Purpose of the Study:

  • To review the advancements in Cell-SELEX technology.
  • To discuss the advantages of using live cells as targets in aptamer selection.
  • To highlight the potential of Cell-SELEX in molecular biology and medical applications.

Main Methods:

  • Cell-SELEX involves iterative selection and amplification of nucleic acid libraries against whole live cells.
  • This method allows for the selection of aptamers that recognize targets in their native conformation on the cell surface.
  • Cell-SELEX can identify cell-specific aptamers without prior knowledge of the target cell's surface molecules.

Main Results:

  • Cell-SELEX generates aptamers functional against native target conformations on live cells.
  • This approach is particularly useful for targeting cell surface transmembrane proteins that are difficult to purify.
  • The technology enables the isolation of cell-specific aptamers, even when the identity of surface markers is unknown.

Conclusions:

  • Cell-SELEX offers significant advantages over traditional SELEX by utilizing live cells as targets.
  • It facilitates the development of aptamers for challenging targets, including those on cell surfaces.
  • The technology holds promise for advancing molecular biology research and developing novel medical diagnostics and therapeutics.