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Updated: May 13, 2026
Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
Published on: September 28, 2022
Aqueous microwave-assisted solid-phase peptide synthesis using fmoc strategy. II. Racemization studies and water
Keiko Hojo1, Natsuki Shinozaki, Asaki Hara
1Faculty of Pharmaceutical Sciences & Cooperative Research Center of Life Sciences, Kobe Gakuin University, 1-1-3 Minatojima, Chuo-ku, Kobe 650-8586, Japan. hojo@pharm.kobegakuin.ac.jp
Abstract:
We have developed a microwave (MW)-assisted peptide synthesis using Fmoc-amino acid nanoparticles in water previously. It is an organic solvent-free, environmentally friendly method for peptide synthesis. In this study, we have investigated the racemization of cysteine during an aqueous based coupling reaction with MW irradiation. Under our MW-assisted protocol using WSCI and DMTMM, the coupling reaction can be performed with low levels of racemization of cysteine. We also demonstrated the synthesis of the nonapeptide oxytocin analogue, Cys(Acm)-Tyr-Ile-Gln-Asn- Cys(Acm)-Pro-Leu-Gly-NH2 using our water based MW-assisted protocol with Fmoc-amino acid nanoparticles.
