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Updated: May 12, 2026

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

Published on: December 28, 2015

Mammalian expression systems and transfection techniques.

Daunia Laurenti¹, Lezanne Ooi

¹Illawarra Health and Medical Research Institute, School of Biological Sciences, University of Wollongong, Wollongong, NSW, Australia.

Methods in Molecular Biology (Clifton, N.J.)

|March 27, 2013

Summary

This summary is machine-generated.

Studying single ion channels requires delivering DNA into mammalian cells. This chapter explores cationic lipid-based transfection and adenoviral delivery for expressing ion channel genes.

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Area of Science:

Molecular biology
Cell biology
Biophysics

Background:

Ion channels are crucial for cellular signaling pathways.
Understanding individual ion channel function requires isolating their effects.
Mammalian cell lines can be engineered to express specific genes.

Purpose of the Study:

To explore methods for delivering DNA into mammalian cells for gene expression.
To focus on techniques suitable for large genes, such as ion channel genes.
To compare lipid-based and viral vector-mediated gene delivery.

Main Methods:

Utilizing cationic lipids to form liposomes for DNA delivery.
Employing viral vectors, specifically adenoviruses, for gene transfer.
Transfecting mammalian cells with plasmid DNA encoding ion channel genes.

Main Results:

Cationic lipid-based transfection facilitates DNA entry into cells.
Adenoviral vectors efficiently deliver genetic material into mammalian cells.
Both methods enable the expression of large genes, including ion channels.

Conclusions:

Gene delivery techniques are essential for studying specific ion channel functions.
Lipid-based and adenoviral methods offer viable strategies for ion channel gene expression in mammalian cells.
These techniques allow for the functional delineation of single ion channel subtypes.