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Updated: May 12, 2026
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
Automated liquid microjunction surface sampling-HPLC-MS/MS analysis of drugs and metabolites in whole-body thin
Vilmos Kertesz1, Gary J Van Berkel
1Organic & Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6131, USA. kerteszv@ornl.gov
Background:
The aim of this work was to develop a fully automated liquid extraction-based surface sampling system utilizing a commercially available autosampler coupled with HPLC-MS/MS detection.
Results:
Discrete spots selected for droplet-based sampling and automated sample queue generation, for both the autosampler and MS, were enabled by using in-house developed software. In addition, co-registration of spatially resolved sampling positions and HPLC-MS information to generate heat maps of compounds monitored for subsequent data analysis was also available in the software. The system was evaluated with whole-body thin tissue sections from propranolol-dosed rats.
Conclusion:
The spatial distributions of both the drug and its hydroxypropranolol glucuronide metabolites were consistent with previous studies employing other liquid extraction-based surface sampling methodologies.
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