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A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer (SMPS-ICPMS)

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Ambient aerodynamic desorption/ionization method for microparticle mass measurement.

Caiqiao Xiong¹, Xiaoyu Zhou, Jianing Wang

¹Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing 100190, China.

Analytical Chemistry

|March 29, 2013

Summary

Aerodynamic desorption (AD) offers a simpler, more sensitive method for rapid in situ mass measurement of microparticles. This technique successfully desorbed various particles, including bacteria and cells, for direct ambient analysis.

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Published on: May 22, 2020

Area of Science:

Analytical Chemistry
Mass Spectrometry
Microparticle Analysis

Background:

In situ mass measurement of microparticles is crucial for various scientific fields.
Existing methods like laser-induced acoustic desorption (LIAD) have limitations in sensitivity and complexity.

Purpose of the Study:

To introduce and evaluate a novel ambient desorption/ionization method for microparticle mass analysis.
To demonstrate the capability of aerodynamic desorption (AD) for rapid, in situ measurement of diverse microparticles.

Main Methods:

Developed an aerodynamic desorption (AD) source utilizing a discontinuous atmospheric pressure interface (DAPI) for pulsed airflow.
Applied AD to desorb various microparticles (bacteria, cells, polystyrene, diamond, silica) under ambient conditions.
Integrated the AD source with a quadrupole ion trap (QIT) mass analyzer and charge detector for mass analysis.

Main Results:

Successfully desorbed diverse microparticles, yielding precharged ions whose charge correlated with particle size.
Demonstrated higher sensitivity for AD compared to LIAD, requiring lower particle concentrations.
Achieved successful in situ mass analysis of red blood cells (RBCs) and E. coli bacteria, obtaining their mass and distributions.

Conclusions:

Aerodynamic desorption (AD) is a highly sensitive and simpler alternative for ambient microparticle mass spectrometry.
The AD method allows for direct sampling and analysis of liquid, solid, and gaseous samples under ambient conditions.
AD provides a viable tool for rapid, in situ characterization of microparticles, including biological entities.