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Published on: May 7, 2018
Construction and expression of an eukaryotic expression vector containing the IER3 gene
Zhen Wang1, Hong-Sheng Yu, Ru-Yong Yao
1Department of Oncology, Affiliated Hospital of Medical College, Qingdao University, Qingdao, China.
Asian Pacific Journal of Cancer Prevention : APJCP
|March 29, 2013
Summary
Researchers successfully created an expression vector for the immediately early response gene 3 (IER3). This tool aids in studying IER3
Area of Science:
- Molecular Biology
- Gene Expression Analysis
Background:
- The immediately early response gene 3 (IER3) is implicated in critical biological processes including apoptosis, immunoreaction, viral infection, and cancer development.
- Understanding IER3's role is crucial for advancing research in these areas.
Purpose of the Study:
- To construct a functional eukaryotic expression vector for the immediately early response gene 3 (IER3).
- To confirm the successful expression of the IER3 gene in a cellular model.
Main Methods:
- Construction of an eukaryotic expression vector containing the IER3 gene.
- Transfection of A549 cells with the constructed vector.
- Assessment of gene expression using fluorescence microscopy and Western blotting.
Main Results:
- Successful expression of IER3 in transfected A549 cells was confirmed.
- Fluorescence microscopy indicated high GFP levels in the cytoplasm at 48 and 72 hours post-transfection.
- Western blotting demonstrated a significant increase in IER3 gene expression compared to control groups.
Conclusions:
- The developed recombinant plasmid expression vector for IER3 is functional.
- This vector provides a valuable tool for further investigating the role of IER3, particularly in the context of lung cancer.
