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Published on: April 16, 2015
BATF regulates the development and function of IL-17 producing iNKT cells
Kimberly L Jordan-Williams1, Stacie Poston, Elizabeth J Taparowsky
1Department of Biological Sciences and Purdue University Center for Cancer Research, Purdue University, West Lafayette, IN 47907, USA.
BMC Immunology
|March 30, 2013
Summary
The transcription factor BATF regulates IL-17 production in immune cells. While BATF promotes Th17 cell expansion, it restricts iNKT cell numbers, skewing them toward an IL-17-producing phenotype.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Basic leucine zipper transcription factor, ATF-activating response element (BATF) is crucial for immune system function.
- Batf null mice exhibit deficiencies in CD4+ Th17 cells, T follicular helper cells, and B cell class switching.
- This study investigates the role of BATF in the development and function of IL-17-producing invariant natural killer T (iNKT-17) cells.
Purpose of the Study:
- To determine the impact of altered BATF expression on iNKT-17 cell development and function.
- To elucidate the molecular mechanisms by which BATF influences IL-17 production in different T cell lineages.
Main Methods:
- Utilized transgenic (Tg) mice overexpressing BATF in T cells and Batf null mice.
- Analyzed iNKT cell populations in vivo and in vitro.
- Investigated gene expression related to IL-17 production.
Main Results:
- BATF directly impacts IL-17 expression in iNKT cells.
- BATF overexpression restricts iNKT cell numbers but promotes an iNKT-17 phenotype.
- In contrast to Th17 cells, BATF overexpression leads to reduced iNKT cell expansion despite promoting IL-17 production.
Conclusions:
- BATF is a molecular signature for IL-17-producing cells, alongside RORγt.
- BATF represents a potential therapeutic target for regulating IL-17-mediated diseases.
- BATF differentially regulates iNKT-17 and Th17 cell expansion, despite targeting the Il17a gene in both.
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