Related Experiment Video
Updated: May 12, 2026
14:14
Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis
Published on: April 29, 2007
Fibrinogen
Linda J Stang1, Lesley G Mitchell
1Department of Laboratory Medicine and Pathology, University of Alberta Hospital, Edmonton, AB, Canada.
Methods in Molecular Biology (Clifton, N.J.)
|April 3, 2013
Summary
The Clauss fibrinogen assay is a key laboratory method for measuring fibrinogen, a crucial protein in blood clotting. This functional assay quantifies fibrinogen
Area of Science:
- Hematology
- Clinical Chemistry
Background:
- Fibrinogen is essential for blood clot formation, acting as the final substrate in the coagulation cascade.
- Laboratory measurement of fibrinogen is critical for assessing hemostasis and diagnosing bleeding disorders.
Purpose of the Study:
- To detail the methodology and principles of the Clauss fibrinogen assay.
- To provide an overview of alternative fibrinogen measurement techniques.
Main Methods:
- The Clauss assay is a quantitative, clot-based functional test.
- It measures fibrinogen's ability to form a clot upon addition of thrombin to diluted plasma.
- Clotting time is measured and compared to a standard curve.
Main Results:
- The Clauss assay is the most common method for fibrinogen quantification.
- Pre-dilution of plasma minimizes interference from heparin and fibrinogen degradation products.
- Alternative methods include prothrombin time-derived and antigenic assays.
Conclusions:
- The Clauss fibrinogen assay is a reliable method for determining functional fibrinogen levels.
- Understanding different fibrinogen assays aids in accurate coagulation assessment.
- Accurate fibrinogen measurement is vital for patient care.
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